bj fibroblast cells  (ATCC)

Journal: Carbohydrate polymers

Article Title: Nitric oxide-releasing dextran surface with enhanced albumin affinity mitigates infection and foreign body reaction

doi: 10.1016/j.carbpol.2025.124855

Figure Lengend Snippet: Biocompatibility evaluation of surfaces. (A) Surface adsorbed bovine serum albumin after 90 mins incubation at physiology conditions ( N > 4). (B) Indirect contact cytotoxicity screening of sample groups against BJ human fibroblast cells (24h) showed the viability of cells across all sample groups tested ( N > 5). (C) Percent hemolysis of the film samples (N > 4). 2–5 % hemolytic activity is considered slightly hemolytic, and > 5 % activity is considered hemolytic according to ASTM F756. Error bars represent standard deviation. **** represents statistical significance ( p < 0.0001).

Article Snippet: BJ fibroblast cells (BJ CRL-2522) were derived from stocks previously acquired from the American Type Culture Collection.

Techniques: Incubation, Activity Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Chitosan-dextran sulfate nanocapsules for enhanced tigecycline efficacy against non-typhoidal Salmonella enterica

doi: 10.1038/s41598-026-35229-7

Figure Lengend Snippet: TGC and/or unloaded CD NPs ameliorated the S. Typhimurium infected group induced histopathological alterations in mice’s liver tissues. Representative photomicrographs of the H&E-stained hepatic tissue sections showing the control (A), S. Typhimurium infected group (C), TGC (E), unloaded CD NPs (G), CD-TGC groups (I) and their respective higher magnifications (B, D, F, H, and J). A, B: control group displaying normal central vein (CV), hepatic cords (HC) with hepatocytes of eosinophilic granular cytoplasm (EC), rounded central single (SN) or double vesicular nuclei (DN), and kupffer cells (KC). C, D: S. Typhimurium infected group demonstrating multiple areas of variable-sized necrotic areas (NA) of coagulative necrosis (CN), severely dilated and congested sinusoid (SDS) with Kupffer cell hyperplasia (KCH). E, F: TGC group displaying moderately sized necrotic areas (MNA), moderately dilated and congested sinusoids (MDS), moderately hyperplastic Kupffer’s cells (MKC), interstitial mononuclear cell infiltration (MI), fibroblast proliferation (FP), and regenerated hepatocytes of stippling basophilic cytoplasm (BC) and pale nuclei (PN). G, H: unloaded CD NPs group showing a few scattered minute necrotic areas (mNA), intense mononuclear cell infiltration (IMI) around the portal area, and apparently normal hepatocytes (NH). I, J: CD-TGC group showed normal hepatocytes (NH), few dilated blood vessels (DBV), and a few interstitial lymphocytic aggregates (FL). Scale bars = 100 μm in A, C, E, G, I; and = 20 μm in B, D, F, H, and J. K: Bar charts demonstrate the statistical analysis of the comparative quantification of the hepatic injury scores in all studied groups. Bars carrying different superscript letters (a, b, c, d, and e) are significantly different as analyzed by the one-way ANOVA test, followed by the multiple comparisons by Duncan’s Post-hoc test ( p < 0.05). Values are the mean of 6 mice per group ± S.E.M.

Article Snippet: BJ normal human foreskin primary fibroblast cell line (ATCC CRL-2522) was used for studying safety of CD-TGC nanocapsules.

Techniques: Infection, Staining, Control

Journal: Journal of Molecular Neuroscience

Article Title: Early Reprogramming Intermediates Enable Direct Neuronal Conversion Via NGN2

doi: 10.1007/s12031-025-02460-2

Figure Lengend Snippet: Reprogramming of human fetal fibroblasts in microfluidics generates early intermediate populations undergoing MET remodeling. (a) Schematic experimental strategy. (b) Morphological conversion along the reprogramming process. Circle: cells undergoing mesenchymal-to-epithelial transition (MET). Scale bar 200 μm. (c) UMAP representation of single-cell RNA sequencing data of reprogramming samples collected on days (D) 0, 3, 5, and 11. (d) Left, cluster analysis of single-cell RNA sequencing data. SR: Somatic-related cluster, DR: Developmental-related cluster, NA: non-associated. Right, bar plot showing the distribution of each cluster across different reprogramming time points (D0, D3, D5, and D11). The proportion of SR and DR populations shifts progressively during reprogramming, with SR clusters predominating at early stages and DR clusters emerging at later time points. (e) Expression dynamics and UMAP representation showing the expression of fibroblast (left), transient (centre), and primed (right) pluripotency markers in the reprogramming clusters in single-cell RNA sequencing data of D0-3–5−11

Article Snippet: The reprogramming protocol was performed from human foreskin BJ fibroblasts (ATCC) as previously described (Gagliano et al. ; Panariello et al. ).

Techniques: RNA Sequencing, Expressing